In planetary magnetospheres, singly charged energetic particles, trapped by the planet’s magnetic field, can steal electrons from cold gas atoms and become neutralized. These now Energetic Neutral Atoms (ENAs), no longer confined by the magnetic field, can travel out of the system similar to photons leaving a hot oven. ENA’s have been used to image magnetospheric processes at Earth, Jupiter, and Saturn. At Jupiter, the opportunities to image the magnetosphere have been limited and always from the perspective of the near-equatorial plane at distance >139 RJ. The polar orbiting Juno mission carries the JEDI instrument that is serendipitously sensitive to ENA’s with energies > 50 keV, provided there are no charged particles in the environment to mask their presence. Here we report on the first ENA observations of Jupiter’s magnetosphere from a non-equatorial perspective. In this brief report we concentrate on emissions seen during perijove 22 (PJ22) during very active conditions, and compare them with emissions during the inactive perijove 23 (PJ23). We observe, and discriminate between, distinct ENA signatures from the neutral gases occupying the orbit of Io (away from Io itself), the orbit of Europa (away from Europa), and from Jupiter itself. Strong ENA emissions from Io’s orbit during PJ22 are associated with energetic particle injections observed near Io’s orbit several hours earlier. Some injections occurred planetward of Io’s L-shell (magnetic position), somewhat of a surprise given that injections are thought to be driven by outward transport of plasmas generated by Io.