Published in

Cambridge University Press (CUP), Microscopy and Microanalysis, 1(3), p. 47-52, 1997

DOI: 10.1017/s1431927697970033

Links

Tools

Export citation

Search in Google Scholar

Laser Microdissection for Generation of a Human Chromosome Region-specific Library

Journal article published in 1997 by Wei He, Yagang Liu, Moyra Smith, Michael W. Berns
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

Full text: Unavailable

Green circle
Preprint: archiving allowed
Green circle
Postprint: archiving allowed
Red circle
Published version: archiving forbidden
Data provided by SHERPA/RoMEO

Abstract

A human chromosome pq34 region-specific, microdissected library was constructed by using laser microdissection techniques. This library contains over 10,000 clones with an average insert size of 450 bp. It has greater coverage of the dissected chromosome region as compared with the needle-dissected chromosome 9q34 library. The laser microdissection technique provides more accurate chromosome targeting and easier operation than existing needle microdissection techniques. To simplify the procedure for chromosome microdissection and chromosome fragment collection, a trapping-cutting system was developed. This technique involves the use of two trapping beams which hold a single chromosome in suspension, and a third cutting beam, which dissects the immobilized chromosome. A collection chamber allowing for the fast collection of dissected chromosome fragments needs to be developed. However, DNA can be cloned from trapped chromosome fragments with an insert size comparable to that of both needle-cut and laser-cut chromosomes.

Beta version