Measuring in situ soil fluxes of carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O) continuously at high frequency requires appropriate technology. We tested the combination of a commercial automated soil CO 2 flux chamber system (LI-8100A) with a CH 4 and N 2 O analyzer (Picarro G2308) in a tropical rainforest for 4 months. A chamber closure time of 2 minutes was sufficient for a reliable estimation of CO 2 and CH 4 fluxes (100 % and 98.5 % of fluxes were above Minimum Detectable Flux – MDF, respectively). This closure time was generally not suitable for a reliable estimation of the low N 2 O fluxes in this ecosystem but was sufficient for detecting rare major peak events. A closure time of 25 minutes was more appropriate for reliable estimation of most N 2 O fluxes (85.6 % of measured fluxes are above MDF ± 0.002 nmol m −2 s −1 ). Our study highlights the importance of adjusted closure time for each gas.