Satellite-retrieved Solar Induced Chlorophyll Fluorescence (SIF) has shown great potential to monitor the photosynthetic activity of terrestrial ecosystems. However, several issues, including low spatial and temporal resolution of the gridded datasets and high uncertainty of the individual retrievals, limit the applications of SIF. In addition, inconsistency in measurements footprints also hinder the direct comparison between gross primary production (GPP) from eddy covariance (EC) flux towers and satellite-retrieved SIF. In this study, by training a neural network (NN) with surface reflectance from the MODerate-resolution Imaging Spectroradiometer (MODIS) and SIF from Orbiting Carbon Observatory-2 (OCO-2), we generated two global spatially continuous SIF (CSIF) datasets at moderate spatio-temporal resolutions (0.05 degree 4-day) during 2001–2016, one for clear-sky conditions and the other one in all-sky conditions. The clear-sky instantaneous CSIF (CSIF clear-inst ) shows high accuracy against the clear-sky OCO-2 SIF and little bias across biome types. The all-sky daily average CSIF (CSIF all-daily ) dataset exhibits strong spatial, seasonal and interannual dynamics that are consistent with daily SIF from OCO-2 and the Global Ozone Monitoring Experiment-2 (GOME-2). An increasing trend (0.39 %) of annual average CSIF all-daily is also found, confirming the greening of Earth in most regions. Since the difference between satellite observed SIF and CSIF is mostly caused by the environmental down-regulation on SIF yield , the ratio between OCO-2 SIF and CSIF clear-inst can be an effective indicator of drought stress that is more sensitive than normalized difference vegetation index and enhanced vegetation index. By comparing CSIF all-daily with gross primary production (GPP) estimates from 40 EC flux towers across the globe, we find a large cross-site variation (c.v. = 0.36) of GPP-SIF relationship with the highest regression slopes for evergreen needleleaf forest. However, the cross-biome variation is relatively limited (c.v. = 0.15). These two continuous SIF datasets and the derived GPP-SIF relationship enable a better understanding of the spatial and temporal variations of the GPP across biomes and climate.